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Having in mind that the ER-positivity score and patient survival correlate positively [17], and the need for an objective ER quantification, we aimed to establish a procedure for the photometric and morphometric analysis of BC immunohistochemical parameters corresponding to BCs with different biological aggressiveness, that can possibly be used for differential diagnostic purposes that are in line with the current semi-quantitative scoring system. The study was conducted on 66 samples of BC, stained using a standard method for the presence of ER, using a procedure that encompassed image processing and nuclear color intensity quantification. 2.2. Immunohistochemical Staining. Semi-quantitative analysis of ER-stained tissue sections was performed following the Allred scoring system guidelines. To obtain the final scores, individual scores of the percentage of ER-positive cancer cell nuclei (0–5) and the staining intensity of the nuclei (0–3) ( Figure 1 ) were summed up. The percentage of ER-positive cancer cell nuclei was set as follows: 1—less than 1% of positive cancer cell nuclei; 2—from 1 to 10% of positive cancer cell nuclei; 3—from 11 to 33% of positive cancer cell nuclei; 4—from 34 to 66% of positive cancer cell nuclei; and score 5—more than 67% of positive cancer cell nuclei ( Figure 1 ; first addend). Whereas, the staining intensity in the nuclei was scored as: 1—weak; 2—medium; and 3—strong ( Figure 1 ; second addend). Dessa forma, os riscos só são maiores quando se está ganhando, o que acaba sendo menos estressante para quem procura um método menos arriscado.Os meus 3 best bola com Melhores métodos para Investimentos Esportivas. Experimental Scoring System.
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