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Genes were clustered according to phylogenetic relationships in expression profiles. The RPKM values were transformed into Z scores. Z scores were plotted according to Z = (x - μ)/σ, where x is the log 2 transformed gene expression measurement and μ and σ are the mean and standard deviations of expression of the gene. Red and blue colors indicate high and low relative expression levels after homogenization, respectively. The RPKM value for each gene and each stage was provided in the figure. Figure 5. Quantitative RT-PCR examination of 10 β -hsd genes expressions in the brain and gonad during sex reversal of grouper. Values represent the relative mRNA expression in relation to internal control (β -actin gene). Data were expressed as the mean ± SEM for four replicates.
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