Como vendas apostas de futebol

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With the increased postnatal expression of Arhgef7 several shorter variants become more prominent with an isoform of approximately 90 kDa being the most strongly expressed one. Staining of cultured neurons from the hippocampus of E18 rat embryos showed that Arhgef7 is present in the soma and at the tip all neurites of unpolarized stage 2 neurons. Upon neuronal polarization it becomes enriched in the axonal growth cone at stage 3 but is present also in the minor neurites (Fig. 1c). Figure 2. To address the question if Arhgef7 is required for axon formation also in vivo we analyzed the phenotype of a conditional Arhgef7 knockout. Since a complete knockout of Arhgef7 is embryonically lethal 15 we generated a cortex-specific knockout using a conditional Arhgef7 allele ( Arhgef7 flox ; Suppl. Fig. S1) and the Emx1-Cre line, which mediates the deletion in the dorsal telencephalon from E10.5 onwards 47,48 . Western blots confirmed the loss of Arhgef7 in the embryonic cortex of homozygous E17.5 Arhgef7 flox/flox ; Emx1 Cre/+ knockout mice (called Arhgef7-cKO hereafter) (Fig. 3a). We cultured cortical neurons from homozygous or heterozygous ( Arhgef7 flox/+ ; Emx1 Cre/+ ) E17.5 Arhgef7-cKO embryos and analyzed axon formation at 3 d.i.v. by staining with an antibody specific for Arhgef7 and markers for axons (Tau-1) and dendrites (MAP2) (Fig.

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