Figure 1. Chromosome localization of 19 β -hsd genes from orange-spotted grouper. The exon-intron structure of the grouper β -hsd genes was further characterized, and showed that the number of introns in each β -hsd gene varied from 2–16 (Figure 2B). Hsd3b1 was the gene with the lowest number of introns, containing only two, while hsd17b4 had the greatest amount at 16. In addition, a phylogenetic tree was constructed using the grouper β-HSD protein sequences to determine whether the exon-intron structure is consistent with the phylogenetic tree (Figure 2A). As expected, several genes with similar exon/intron structures were clustered together on the phylogenetic tree, such as hsd17b7 / hsd17b7l , hsd20b2 / hsd17b12a / hsd17b12b .
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